LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2159..2255) /label=attL2 /note="attL2 (c)" misc_signal complement(2270..2289) /note="T7 Promoter/priming site (c)" primer_bind 2297..2313 /note="M13 Reverse priming site" gene 2426..3235 /note="Kanamycin resistance gene" rep_origin 3356..4029 /note="pUC origin" PCR_frame 669..2159 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2179) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2176) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2167..4032,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2137..2157 /note="TEV site" source 673..2136 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:3136 IMAGE:3353085" /tissue_type="Eye, retinoblastoma" /clone_lib="NIH_MGC_16" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2136 /gene="ALG12" /gene_synonym=ECM39 /gene_synonym=hALG12 /gene_synonym=PP14673 /db_xref="GeneID:79087" /db_xref="HGNC:19358" /db_xref="MIM:607144" CDS 673..2136 /dnas_title="asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae)" /gene="ALG12" /gene_synonym=ECM39 /gene_synonym=hALG12 /gene_synonym=PP14673 /codon_start=1 /product="asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae)" /protein_id="AAH01729.1" /db_xref="GI:12804615" /db_xref="GeneID:79087" /db_xref="HGNC:19358" /db_xref="MIM:607144" /translation="MAGKGSSGRRPLLLGLLVAVATVHLVICPYTKVEESFNLQATHD LLYHWQDLEQYDHLEFPGVVPRTFLGPVVIAVFSSPAVYVLSLLEMSKFYSQLIVRGV LGLGVIFGLWTLQKEVRRHFGAMVATMFCWVTAMQFHLMFYCTRTLPNVLALPVVLLA LAAWLRHEWARFIWLSAFAIIVFRVELCLFLGLLLLLALGNRKVSVVRALRHAVPAGI LCLGLTVAVDSYFWRQLTWPEGKVLWYNTVLNKSSNWGTSPLLWYFYSALPRGLGCSL LFIPLGLVDRRTHAPTVLALGFMALYSLLPHKELRFIIYAFPMLNITAARGCSYLLNN YKKSWLYKAGSLLVIGHLVVNAAYSATALYVSHFNYPGGVAMQRLHQLVPPQTDVLLH IDVAAAQTGVSRFLQVNSAWRYDKREDVQPGTGMLAYTHILMEAAPGLLALYRDTHRV LASVVGTTGVSLNLTQLPPFNVHLQTKLVLLERLPRPS" misc_feature 673..2136 /note="ALG12 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggctgg aaaggggtca tcaggcaggc ggcccctgct gctggggctg 721 ctggtggccg tagccactgt ccacctggtc atctgtccct acaccaaagt ggaggagagc 781 ttcaacctgc aggccacaca tgacctgctc taccactggc aagacctgga gcagtacgac 841 catcttgagt tccccggagt cgtccccagg acgttcctcg ggccagtggt gatcgcagtg 901 ttctccagcc ccgcggttta cgtgctttcg ctgttagaaa tgtccaagtt ttactctcag 961 ctaatagtta gaggagtgct tggactcggc gtgatttttg gactctggac gttacaaaag 1021 gaagtgagac ggcacttcgg ggccatggtg gccaccatgt tctgctgggt gacggccatg 1081 cagttccacc tgatgttcta ctgcacgcgg acactgccca atgtgctggc cctgcctgta 1141 gtcctgctgg ccctcgcggc ctggctgcgg cacgagtggg cccgcttcat ctggctgtca 1201 gccttcgcca tcatcgtgtt cagggtggag ctgtgcctgt tcctgggcct cctgctgctg 1261 ctggccttgg gcaaccgaaa ggtttctgta gtcagagccc ttcgccacgc cgtcccggca 1321 gggatcctct gtttaggact gacggttgct gtggactctt atttttggcg gcagctcact 1381 tggccggaag gaaaggtgct ttggtacaac actgtcctga acaaaagctc caactggggg 1441 acctccccgc tgctgtggta cttctactca gccctgcccc gcggcctggg ctgcagcctg 1501 ctcttcatcc ccctgggctt ggtagacaga aggacgcacg cgccgacggt gctggcactg 1561 ggcttcatgg cactctactc cctcctgcca cacaaggagc tacgcttcat catctatgcc 1621 ttccccatgc tcaacatcac ggctgccaga ggctgctcct acctgctgaa taactataaa 1681 aagtcttggc tgtacaaagc ggggtctctg cttgtgatcg gacacctcgt ggtgaatgcc 1741 gcctactcag ccacggccct gtatgtgtcc catttcaact acccaggtgg cgtcgcaatg 1801 cagaggctgc accagctggt gcccccccag acagacgtcc ttctgcacat tgacgtggca 1861 gccgcccaga caggtgtgtc tcggtttctc caagtcaaca gcgcctggag gtacgacaag 1921 agggaggatg tgcagccggg gacaggcatg ctggcataca cacacatcct catggaggcg 1981 gcccctgggc tcctggccct ctacagggac acacaccggg tcctggccag cgtcgtgggg 2041 accacaggtg tgagtctgaa cctgacccaa ctgcccccct tcaacgtcca cctgcagaca 2101 aagctggtgc ttctggagag gctcccccgg ccgtccgaaa acttgtactt tcaaggctCC 2161 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA 2221 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG 2281 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT 2341 CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA ACAATAAAAC TGTCTGCTTA 2401 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG 2461 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG 2521 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT 2581 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG 2641 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC 2701 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC 2761 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT 2821 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC 2881 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC 2941 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT 3001 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG 3061 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA 3121 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA 3181 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA 3241 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG 3301 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT 3361 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 3421 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 3481 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA 3541 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 3601 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 3661 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 3721 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA 3781 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 3841 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 3901 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 3961 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 4021 TGCTCACATG TT //